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Addgene

LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)
(Plasmid #132926)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132926 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221 P4r-P3r
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2664
  • Total vector size (bp) 4443
  • Vector type
    Luciferase, Synthetic Biology ; pMVP Gateway Entry plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luc2P
  • Alt name
    Firefly luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    1779
  • Tag / Fusion Protein
    • PEST destabilization domain (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Luc2P gene was PCR'd from pGL4.27 (Promega).

Entry plasmid is compatible with pMVP cloning platform described in PMID: 30590691.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P) was a gift from Christopher Newgard (Addgene plasmid # 132926 ; http://n2t.net/addgene:132926 ; RRID:Addgene_132926)