LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)
(Plasmid
#132926)
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PurposepMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132926 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221 P4r-P3r
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2664
- Total vector size (bp) 4443
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Vector typeLuciferase, Synthetic Biology ; pMVP Gateway Entry plasmid
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuc2P
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Alt nameFirefly luciferase
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SpeciesSynthetic
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Insert Size (bp)1779
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Tag
/ Fusion Protein
- PEST destabilization domain (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Luc2P gene was PCR'd from pGL4.27 (Promega).
Entry plasmid is compatible with pMVP cloning platform described in PMID: 30590691.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P) was a gift from Christopher Newgard (Addgene plasmid # 132926 ; http://n2t.net/addgene:132926 ; RRID:Addgene_132926)