Gal ts-lux
(Plasmid #1329)

• Depositing Lab: Susan Lindquist
• Publication: Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56.

Backbone

• Vector backbone: pRS316 Gal1-10 (based on pBluescript)
• Backbone size w/o insert (bp): 4895
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: bacterial luciferase-V.h.MAV
• Alt name: Vibrio harveyi luciferase in yeast
• Species: Vibrio harveyi MAV
• Insert Size (bp): 2270

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: blunt (destroyed during cloning)
• 5′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A.Szalay, University of Alberta

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid with selectable Ura marker, maintained in DH5alphaF'

Using PCR, BamH1 and Sac1 sites were created at the end of the luxAB gene (pLx709-fab9) and this wascloned into pRS316 Gal1-10 vector