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Addgene

GFP-NPM NLS2D
(Plasmid #13285)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPM
  • Alt name
    B23.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    Deletion of aa150-aa155
  • Entrez Gene
    NPM1 (a.k.a. B23, NPM)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    K. Nagata (U of Tsukuba, Japan)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NLS2D=nuclear localization signal deletion

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-NPM NLS2D was a gift from Xin Wang (Addgene plasmid # 13285 ; http://n2t.net/addgene:13285 ; RRID:Addgene_13285)

  • For your References section:

    Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. Wang W, Budhu A, Forgues M, Wang XW. Nat Cell Biol. 2005 Aug . 7(8):823-30. 10.1038/ncb1282 PubMed 16041368
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