GFP-NPM NLS2D
(Plasmid
#13285)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C2
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPM
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Alt nameB23.1
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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MutationDeletion of aa150-aa155
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Entrez GeneNPM1 (a.k.a. B23, NPM)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byK. Nagata (U of Tsukuba, Japan)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NLS2D=nuclear localization signal deletion
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-NPM NLS2D was a gift from Xin Wang (Addgene plasmid # 13285 ; http://n2t.net/addgene:13285 ; RRID:Addgene_13285) -
For your References section:
Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. Wang W, Budhu A, Forgues M, Wang XW. Nat Cell Biol. 2005 Aug . 7(8):823-30. 10.1038/ncb1282 PubMed 16041368
Map uploaded by the depositor.