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Addgene

piHGpd/G170D
(Plasmid #1328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303 (based on pBluescript)
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GPD promoter-hsp82 ts mutant (G170D)
  • Alt name
    Yeast Heat Shock Protein 82 temperature-sensitive mutant
  • Alt name
    HSP82
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3800
  • Mutation
    G170D
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid maintained in DH5alpha.
Yeast hsp82 ts mutant expressed from the GPD promoter on a His3 marked integrating plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piHGpd/G170D was a gift from Susan Lindquist (Addgene plasmid # 1328 ; http://n2t.net/addgene:1328 ; RRID:Addgene_1328)

  • For your References section:

    In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56. 10.1073/pnas.94.24.12949 PubMed 9371781
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