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Addgene

pDRF1-GW yAT1.03
(Plasmid #132781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDRF1-GW
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    yAT1.03 ymTq2-tdTomato
  • Species
    B. Subtilis

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI, SpeI, XbaI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer AACAATCGTTAATAATTAATTAATTGG
  • 3′ sequencing primer GAGTCACTTTAAAATTTGTATACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRF1-GW yAT1.03 was a gift from Bas Teusink (Addgene plasmid # 132781 ; http://n2t.net/addgene:132781 ; RRID:Addgene_132781)

  • For your References section:

    An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions. Botman D, van Heerden JH, Teusink B. ACS Sens. 2020 Mar 27;5(3):814-822. doi: 10.1021/acssensors.9b02475. Epub 2020 Mar 3. 10.1021/acssensors.9b02475 PubMed 32077276
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