pGEX-4T1-14-3-3 zeta GST
(Plasmid #13278)

• Depositing Lab: Michael Yaffe
• Publication: Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 14-3-3 zeta
• Alt name: YWHAZ
• Species: B. taurus (bovine)
• Entrez Gene: YWHAZ
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI? (unknown if destroyed)
• 3′ cloning site: BamHI? (unknown if destroyed)
• 5′ sequencing primer: pGEX

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA from Tom Roberts.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert PCR'd and cloned into pGEX-4T1 (via either EcoRI and/or BamHI?) by Tom Roberts lab.

Uses stop codons of pGEX backbone rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.