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pU6-pegRNA-GG-acceptor
(Plasmid #132777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Pick pink/red colonies. Growth at 30C helps promote plasmid stability.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Exchangeable cassette
  • Alt name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    821
  • Mutation
    See manuscript
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BsaI sites are located at positions 245 and 1070.
Please note that bacterial colonies for this plasmid often appear pink or red in color because of expression of the RFP insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-pegRNA-GG-acceptor was a gift from David Liu (Addgene plasmid # 132777 ; http://n2t.net/addgene:132777 ; RRID:Addgene_132777)

  • For your References section:

    Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. 10.1038/s41586-019-1711-4 PubMed 31634902
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