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Addgene

pCMV-PE2
(Plasmid #132775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2
  • Alt name
    Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)
  • Species
    Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    6354
  • Mutation
    See manuscript
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on backbone)
    • SV40 NLS (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7-forward
  • 3′ sequencing primer BGH-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PE2, PE3, and PE3b all use this plasmid (pCMV-PE2)
PE3 and PE3b add an sgRNA, which can be any guide RNA expression vector or cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PE2 was a gift from David Liu (Addgene plasmid # 132775 ; http://n2t.net/addgene:132775 ; RRID:Addgene_132775)

  • For your References section:

    Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. 10.1038/s41586-019-1711-4 PubMed 31634902

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