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pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro
(Plasmid #132773)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lamin A
  • Species
    H. sapiens (human)
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro was a gift from Jan Lammerding (Addgene plasmid # 132773 ; http://n2t.net/addgene:132773 ; RRID:Addgene_132773)

  • For your References section:

    Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells. Earle AJ, Kirby TJ, Fedorchak GR, Isermann P, Patel J, Iruvanti S, Moore SA, Bonne G, Wallrath LL, Lammerding J. Nat Mater. 2019 Dec 16. pii: 10.1038/s41563-019-0563-5. doi: 10.1038/s41563-019-0563-5. 10.1038/s41563-019-0563-5 PubMed 31844279
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