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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
(Plasmid #132771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclic GMP-AMP synthase
  • Species
    H. sapiens (human)
  • Mutation
    E225A D227A
  • Entrez Gene
    CGAS (a.k.a. C6orf150, D4, MB21D1, h-cGAS)
  • Tag / Fusion Protein
    • mCherry2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro was a gift from Jan Lammerding (Addgene plasmid # 132771 ; http://n2t.net/addgene:132771 ; RRID:Addgene_132771)

  • For your References section:

    Nuclear envelope rupture and repair during cancer cell migration. Denais CM, Gilbert RM, Isermann P, McGregor AL, te Lindert M, Weigelin B, Davidson PM, Friedl P, Wolf K, Lammerding J. Science. 2016 Apr 15;352(6283):353-8. doi: 10.1126/science.aad7297. Epub 2016 Mar 24. 10.1126/science.aad7297 PubMed 27013428
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