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Addgene

pGEX-2TK-14-3-3 eta GST
(Plasmid #13277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 eta
  • Species
    H. sapiens (human)
  • Entrez Gene
    YWHAH (a.k.a. YWHA1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pGEX
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2TK-14-3-3 eta GST was a gift from Michael Yaffe (Addgene plasmid # 13277 ; http://n2t.net/addgene:13277 ; RRID:Addgene_13277)

  • For your References section:

    The structural basis for 14-3-3:phosphopeptide binding specificity. Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, Gamblin SJ, Smerdon SJ, Cantley LC. Cell. 1997 Dec 26. 91(7):961-71. 10.1016/S0092-8674(00)80487-0 PubMed 9428519
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