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Purposeapplication in Correlative light-electron microscopy
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelamin A
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SpeciesH. sapiens (human)
- Promoter CMV
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Tag
/ Fusion Protein
- mEosEM (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEosEM-Lamin A was a gift from Pingyong Xu (Addgene plasmid # 132707 ; http://n2t.net/addgene:132707 ; RRID:Addgene_132707) -
For your References section:
mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM. Fu Z, Peng D, Zhang M, Xue F, Zhang R, He W, Xu T, Xu P. Nat Methods. 2019 Oct 14. pii: 10.1038/s41592-019-0613-6. doi: 10.1038/s41592-019-0613-6. 10.1038/s41592-019-0613-6 PubMed 31611693