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Addgene

Mito-mEosEM
(Plasmid #132706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mitochondria targeting sequence
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEosEM (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mito-mEosEM was a gift from Pingyong Xu (Addgene plasmid # 132706 ; http://n2t.net/addgene:132706 ; RRID:Addgene_132706)

  • For your References section:

    mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM. Fu Z, Peng D, Zhang M, Xue F, Zhang R, He W, Xu T, Xu P. Nat Methods. 2019 Oct 14. pii: 10.1038/s41592-019-0613-6. doi: 10.1038/s41592-019-0613-6. 10.1038/s41592-019-0613-6 PubMed 31611693
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