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Addgene

pTP223
(Plasmid #13263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMC7
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plac-gam-bet-exo
  • Alt name
    Lambda Red
  • Species
    bacteriophage lambda
  • Insert Size (bp)
    2000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Anthony Poteete
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original description can be found in Poteete & Fenton (1984) Virology, 134:161-167.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP223 was a gift from Kenan Murphy (Addgene plasmid # 13263 ; http://n2t.net/addgene:13263 ; RRID:Addgene_13263)

  • For your References section:

    Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy KC, Campellone KG. BMC Mol Biol. 2003 Dec 13. 4():11. 10.1186/1471-2199-4-11 PubMed 14672541
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