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Addgene

pAL- S. pombe TSC1 (1-431)
(Plasmid #132616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TSC1 (1-431)
  • Species
    S. pombe (fission yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the plasmid contains a longer TSC1 (1-899) variant that contains the stop codon at 432 to generate the TSC1 (1-431) variant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL- S. pombe TSC1 (1-431) was a gift from Wenqing Xu (Addgene plasmid # 132616 ; http://n2t.net/addgene:132616 ; RRID:Addgene_132616)

  • For your References section:

    Crystal structure of the yeast TSC1 core domain and implications for tuberous sclerosis pathological mutations. Sun W, Zhu YJ, Wang Z, Zhong Q, Gao F, Lou J, Gong W, Xu W. Nat Commun. 2013;4:2135. doi: 10.1038/ncomms3135. 10.1038/ncomms3135 PubMed 23857276
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