pGEX4T-1 human RNF146 WWE (99-183)
(Plasmid
#132613)
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Purposeexpresses human RNF146 WWE (99-183) in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRNF146 WWE (99-183)
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SpeciesH. sapiens (human)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T-1 human RNF146 WWE (99-183) was a gift from Wenqing Xu (Addgene plasmid # 132613 ; http://n2t.net/addgene:132613 ; RRID:Addgene_132613) -
For your References section:
Recognition of the iso-ADP-ribose moiety in poly(ADP-ribose) by WWE domains suggests a general mechanism for poly(ADP-ribosyl)ation-dependent ubiquitination. Wang Z, Michaud GA, Cheng Z, Zhang Y, Hinds TR, Fan E, Cong F, Xu W. Genes Dev. 2012 Feb 1;26(3):235-40. doi: 10.1101/gad.182618.111. Epub 2012 Jan 19. 10.1101/gad.182618.111 PubMed 22267412