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pGEX4T-1-human RNF146 RING-WWE (30-183)
(Plasmid #132611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNF146 RING-WWE (30–183)
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-1-human RNF146 RING-WWE (30-183) was a gift from Wenqing Xu (Addgene plasmid # 132611 ; http://n2t.net/addgene:132611 ; RRID:Addgene_132611)

  • For your References section:

    Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal. DaRosa PA, Wang Z, Jiang X, Pruneda JN, Cong F, Klevit RE, Xu W. Nature. 2015 Jan 8;517(7533):223-6. doi: 10.1038/nature13826. Epub 2014 Oct 19. 10.1038/nature13826 PubMed 25327252
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