phNFL-Q332P
(Plasmid #132599)

• Purpose: Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation
• Depositing Lab: Anthony Brown
• Publication: Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 10.1002/cm.21566. Epub 2019 Nov 6.

Backbone

• Vector backbone: pEGFP-C1 with EGFP sequence removed
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4001
• Modifications to backbone: Obtained by site-directed mutagenesis of the phNFL
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nefl
• Alt name: neurofilament protein L
• Species: H. sapiens (human)
• Insert Size (bp): 2062
• Mutation: 995A>C;Gln332Pro
• Entrez Gene: Nefl (a.k.a. NF-L, NF68, Nfl)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry