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Addgene

prNFL-P8R-EGFP
(Plasmid #132598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4698
  • Modifications to backbone
    Obtained by site-directed mutagenesis of the prNFL-EGFP plasmid. There is a silent mutation at nucleotide 1590G>A.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nefl
  • Alt name
    neurofilament protein L
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1656
  • Mutation
    23C>G; Pro8Arg
  • Entrez Gene
    Nefl (a.k.a. NF-L, NF68, Nfl)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prNFL-P8R-EGFP was a gift from Anthony Brown (Addgene plasmid # 132598 ; http://n2t.net/addgene:132598 ; RRID:Addgene_132598)

  • For your References section:

    Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments. Stone EJ, Uchida A, Brown A. Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 10.1002/cm.21566. Epub 2019 Nov 6. 10.1002/cm.21566 PubMed 31574566
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