Skip to main content
Addgene

phNFL
(Plasmid #132589)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1 with EGFP sequence removed
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4001
  • Modifications to backbone
    EGFP sequence removed using AgeI and BspEI. Kozak sequence immediately 5' of coding region.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEFL
  • Alt name
    neurofilament protein L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2062
  • Entrez Gene
    NEFL (a.k.a. CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The human NFL cDNA was provided by Shin-Ichi Hisanaga.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phNFL was a gift from Anthony Brown (Addgene plasmid # 132589 ; http://n2t.net/addgene:132589 ; RRID:Addgene_132589)

  • For your References section:

    Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments. Stone EJ, Uchida A, Brown A. Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 10.1002/cm.21566. Epub 2019 Nov 6. 10.1002/cm.21566 PubMed 31574566
Related items:
Commonly requested with: