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Addgene

pLentiGuide-Puro/CD73-gRNA4
(Plasmid #132588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiGuide-Puro
  • Backbone size w/o insert (bp) 8298
  • Total vector size (bp) 8323
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD73
  • Alt name
    NT5E
  • gRNA/shRNA sequence
    CACCGTAGTCACTTCTGATGATGGG
  • Species
    H. sapiens (human)
  • GenBank ID
    4907
  • Entrez Gene
    NT5E (a.k.a. CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CACCGTAGTCACTTCTGATGATGGG
  • 3′ sequencing primer AAACCCCATCATCAGAAGTGACTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiGuide-Puro/CD73-gRNA4 was a gift from Robert Sobol (Addgene plasmid # 132588 ; http://n2t.net/addgene:132588 ; RRID:Addgene_132588)

  • For your References section:

    Extracellular NAD(+) enhances PARP-dependent DNA repair capacity independently of CD73 activity. Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, Ferraris DM, Sykora P, Andrews J, Clark J, Davis A, Chaloin L, Rizzi M, Migaud M, Sobol RW. Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9. 10.1038/s41598-020-57506-9 PubMed 31959836
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