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Addgene

MYH11-gRNA1
(Plasmid #132587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSTBlue-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYH11-gRNA1
  • gRNA/shRNA sequence
    AAGGCACCATGGCGCAGAA
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYH11-gRNA1 was a gift from James Thomson (Addgene plasmid # 132587 ; http://n2t.net/addgene:132587 ; RRID:Addgene_132587)

  • For your References section:

    A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia. Zhang J, McIntosh BE, Wang B, Brown ME, Probasco MD, Webster S, Duffin B, Zhou Y, Guo LW, Burlingham WJ, Kent C, Ferris M, Thomson JA. Stem Cell Reports. 2019 Jun 11;12(6):1269-1281. doi: 10.1016/j.stemcr.2019.04.013. Epub 2019 May 9. 10.1016/j.stemcr.2019.04.013 PubMed 31080110
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