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Addgene

pIM04
(Plasmid #132584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZS2
  • Backbone size w/o insert (bp) 3289
  • Total vector size (bp) 5032
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    pLsoxS-TEVp-TermT1
  • Insert Size (bp)
    966
  • Promoter pLsoxS

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCAAGAAAGCCATCCAGTTT
  • 3′ sequencing primer CAGATAGGGACGACGTGGTGTTAGCTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    proD-soxR-TermT1
  • Insert Size (bp)
    777
  • Promoter proD

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGGCCATAAAGTTTTCATGGTGAAACCTGAA
  • 3′ sequencing primer CGATGCTGCTTATCGAATCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIM04 was a gift from Timothy Lu (Addgene plasmid # 132584 ; http://n2t.net/addgene:132584 ; RRID:Addgene_132584)

  • For your References section:

    Gene networks that compensate for crosstalk with crosstalk. Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK. Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y. 10.1038/s41467-019-12021-y PubMed 31492904
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