pIM16
(Plasmid #132583)

• Purpose: pZA4-oxySp-mCherry-pLsoxS-mCherry
• Depositing Lab: Timothy Lu
• Publication: Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.

Backbone

• Vector backbone: pZA4
• Backbone size w/o insert (bp): 2055
• Total vector size (bp): 3984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: oxySp-mCherry-TermT1
• Insert Size (bp): 980
• Promoter: oxySp

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCGAACAGGCTTATGTCCAC
• 3′ sequencing primer: GGTCAGTGCGTCCTGCTGATGTTG

Gene/Insert 2

• Gene/Insert name: pLsoxS-mCherry-TermT1
• Insert Size (bp): 949
• Promoter: pLsoxS

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTCGAGCCATAAATCGCTTTACCTCAAGTT
• 3′ sequencing primer: TGGCATCTTCCAGGAAATCT

Resource Information

• Supplemental Documents:
  • pIM16.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry