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MYH11-NanoLuc-2A-tdTomato
(Plasmid #132569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDNR
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human MYH11 knockin homology arms
  • Alt name
    AAT4, FAA4, SMHC, SMMHC
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYH11 (a.k.a. AAT4, FAA4, SMHC, SMMHC, SMMS-1, VSCM2)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYH11-NanoLuc-2A-tdTomato was a gift from James Thomson (Addgene plasmid # 132569 ; http://n2t.net/addgene:132569 ; RRID:Addgene_132569)

  • For your References section:

    A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia. Zhang J, McIntosh BE, Wang B, Brown ME, Probasco MD, Webster S, Duffin B, Zhou Y, Guo LW, Burlingham WJ, Kent C, Ferris M, Thomson JA. Stem Cell Reports. 2019 Jun 11;12(6):1269-1281. doi: 10.1016/j.stemcr.2019.04.013. Epub 2019 May 9. 10.1016/j.stemcr.2019.04.013 PubMed 31080110
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