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Addgene

pLPC FLAG hRap1 delta Br delta M
(Plasmid #13256)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Repressor Activator Protein 1 delta Br delta M
  • Alt name
    Rap1 delta Br delta M
  • Alt name
    Rap1
  • Alt name
    1102
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    770
  • Mutation
    contains hRap1 aa2-44 + 184-399 and the point mutation M5L
  • GenBank ID
    AF262988
  • Entrez Gene
    TERF2IP (a.k.a. DRIP5, RAP1)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC FLAG hRap1 delta Br delta M was a gift from Titia de Lange (Addgene plasmid # 13256 ; http://n2t.net/addgene:13256 ; RRID:Addgene_13256)

  • For your References section:

    Rap1 affects the length and heterogeneity of human telomeres. Li B, de Lange T. Mol Biol Cell. 2003 Dec . 14(12):5060-8. 10.1091/mbc.e03-06-0403 PubMed 14565979
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