pdCas9-sgRNA-com-BoxB-vector
(Plasmid #132556)

• Purpose: Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
• Depositing Lab: Baisong Lu
• Publication: Lentiviral capsid packaging of spCas9 RNP (unpublished)

Backbone

• Vector backbone: pCDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dCas9-DMNT3a-DMNT3l
• Species: Synthetic
• Entrez Gene: DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)

Gene/Insert 2

• Gene/Insert name: Lambda N22-KRAB
• Species: Synthetic

Gene/Insert 3

• Gene/Insert name: sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • Snapgene file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry