pspCas9-HF-tetra-com-vector
(Plasmid #132555)

• Purpose: Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
• Depositing Lab: Baisong Lu
• Publication: Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/crispr.2020.0106.

Backbone

• Vector backbone: pCDNA3,1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: High fidelity Cas9
• Species: Synthetic

Gene/Insert 2

• Gene/Insert name: sgRNA scaffold with com replacing the Tetraloop
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • snapgene file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry