pspCas9-3'UTR-Tetra-com-vector
(Plasmid
#132553)
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PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 132553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCas9
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SpeciesSynthetic
Gene/Insert 2
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Gene/Insert namesgRNA scaffold with com replacing the Tetraloop
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pspCas9-3'UTR-Tetra-com-vector was a gift from Baisong Lu (Addgene plasmid # 132553 ; http://n2t.net/addgene:132553 ; RRID:Addgene_132553) -
For your References section:
Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing. Lu Z, Yao X, Lyu P, Yadav M, Yoo K, Atala A, Lu B. CRISPR J. 2021 Mar 16. doi: 10.1089/crispr.2020.0106. 10.1089/crispr.2020.0106 PubMed 33733873
