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Superti-Furga Lab Human SLC Knockout Library
(Pooled Library #132552)

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  • Purpose

    The CRISPR knockout library targets the human solute carrier family of proteins. A set of additional 20 essential genes is also targeted as a control set.

  • Vector Backbone

    lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 132552 gRNA pooled library 1 $380 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted

    390 SLCs
    20 essential genes
    7 pseudogenes

  • gRNAs
    2,609
  • Controls
    120 non-targeting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
    Industry Terms
    • Not Available to Industry
    Trademarks
    • Zeocin® is an InvivoGen trademark.

Depositor Comments

The lentiCRISPRv2 vector contains U6 promoter, sgRNA, EFS, SpCas9, FLAG, P2A, puromycin resistance and WPRE. The library contains 2369 sgRNAs targeting 390 solute carriers, 120 sgRNAs targeting 20 essential genes leading to cell death, and 120 non-targeting sgRNAs. The library is packaged in lentivirus to construct a pooled lentiviral library.

Figure 1. Superti-Furga Lab Human CRISPR SLC Knockout Library

The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.

The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human SLC Knockout Library was a gift from Giulio Superti-Furga (Addgene #132552)

  • For your References section:

    A widespread role for SLC transmembrane transporters in resistance to cytotoxic drugs. Girardi E, Cesar-Razquin A, Lindinger S, Papakostas K, Konecka J, Hemmerich J, Kickinger S, Kartnig F, Gurtl B, Klavins K, Sedlyarov V, Ingles-Prieto A, Fiume G, Koren A, Lardeau CH, Kumaran Kandasamy R, Kubicek S, Ecker GF, Superti-Furga G. Nat Chem Biol. 2020 Apr;16(4):469-478. doi: 10.1038/s41589-020-0483-3. Epub 2020 Mar 9. PubMed 32152546
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