AAV-U6-gRNA-TnT-Cre
(Plasmid #132551)

• Purpose: For loss-of-function experiments in cardiomyocytes
• Depositing Lab: William Pu
• Publication: CASAAV single gRNA vector (unpublished)

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 2896
• Total vector size (bp): 5597
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TnT-Cre
• Insert Size (bp): 1797
• Promoter: TnT

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGCACCGAGTCGGTGCTT
• 3′ sequencing primer: GCACTGGGGAGGGGTCACAG

Gene/Insert 2

• Gene/Insert name: U6-gRNA
• Insert Size (bp): 349
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGCGTGAGGGCCTATTTCC
• 3′ sequencing primer: CGCTTCGGACCCGACCCTCT

Resource Information

• Supplemental Documents:
  • AAV-U6-gRNA-TnT-Cre.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry