Dynein-2_complex
(Plasmid #132536)

• Purpose: Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
• Depositing Lab: Andrew Carter
• Publication: Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: Geneva Biotech
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 31586
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: DYNC2H1
• Alt name: Dynein 2 Heavy chain 1
• Alt name: DHC2
• Species: H. sapiens (human)
• Insert Size (bp): 12921
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
• Tags / Fusion Proteins:
  • 8x His (N terminal on insert)
  • ZZ (N terminal on insert)
  • Linker-TEV-linker-TEV (N terminal on insert)
  • SNAPf (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: DYNC2LI1
• Alt name: LIC3
• Alt name: Cytoplasmic Dynein 2 Light Intermediate Chain 1
• Alt name: D2LIC
• Species: H. sapiens (human)
• Insert Size (bp): 1056
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNC2LI1 (a.k.a. CGI-60, D2LIC, LIC3)

Gene/Insert 3

• Gene/Insert name: WDR34
• Alt name: WD repeat-containing protein 34
• Species: H. sapiens (human)
• Insert Size (bp): 1695
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: WDR34 (a.k.a. DIC5, FAP133, SRTD11, bA216B9.3)
• Tag / Fusion Protein:
  • Strep (C terminal on insert)

Gene/Insert 4

• Gene/Insert name: WDR60
• Alt name: WD repeat-containing protein 60
• Species: H. sapiens (human)
• Insert Size (bp): 3201
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: WDR60 (a.k.a. FAP163, SRPS6, SRTD8)

Gene/Insert 5

• Gene/Insert name: DYNLL1
• Alt name: LC8-1
• Alt name: Dynein Light Chain LC8-Type 1
• Alt name: HDLC1
• Species: H. sapiens (human)
• Insert Size (bp): 270
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLL1 (a.k.a. DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1)

Gene/Insert 6

• Gene/Insert name: DYNLL2
• Alt name: LC8-2
• Alt name: Dynein Light Chain LC8-Type 2
• Alt name: DLC2
• Species: H. sapiens (human)
• Insert Size (bp): 270
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLL2 (a.k.a. DNCL1B, Dlc2, RSPH22)

Gene/Insert 7

• Gene/Insert name: DYNLRB1
• Alt name: Rbl-1
• Alt name: Roadblock-1
• Alt name: RB-1
• Insert Size (bp): 291
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLRB1 (a.k.a. BITH, BLP, DNCL2A, DNLC2A, ROBLD1)

Gene/Insert 8

• Gene/Insert name: DYNLRB2
• Alt name: Roadblock-2
• Alt name: Rbl-2
• Alt name: RB-2
• Species: H. sapiens (human)
• Insert Size (bp): 291
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLRB2 (a.k.a. DNCL2B, DNLC2B, ROBLD2)

Gene/Insert 9

• Gene/Insert name: DYNLT1
• Alt name: TCTEX-1
• Species: H. sapiens (human)
• Insert Size (bp): 342
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLT1 (a.k.a. CW-1, TCTEL1, tctex-1)

Gene/Insert 10

• Gene/Insert name: TCTEX1D2
• Alt name: Tctex1 domain-containing protein 2
• Species: H. sapiens (human)
• Insert Size (bp): 429
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: TCTEX1D2 (a.k.a. SRTD17)

Gene/Insert 11

• Gene/Insert name: DYNLT3
• Alt name: TCTEX-3
• Species: H. sapiens (human)
• Insert Size (bp): 351
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNLT3 (a.k.a. RP3, TCTE1L, TCTEX1L)

Gene/Insert 12

• Gene/Insert name: LC8-like
• Alt name: LOC392067
• Species: H. sapiens (human)
• Insert Size (bp): 894
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: LOC392067

Resource Information

• Supplemental Documents:
  • p1742_Dynein-2 complex.gb
• A portion of this plasmid was derived from a plasmid made by: Cloned from genes synthesised by Epoch, codon optised for insect cell expression.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.