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Addgene

DYNLT3
(Plasmid #132532)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132532 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Backbone size w/o insert (bp) 2922
  • Total vector size (bp) 3267
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DYNLT3
  • Alt name
    TCTEX-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    345
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLT3 (a.k.a. RP3, TCTE1L, TCTEX1L)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned from genes synthesised by Epoch, codon optised for insect cell expression.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DYNLT3 was a gift from Andrew Carter (Addgene plasmid # 132532 ; http://n2t.net/addgene:132532 ; RRID:Addgene_132532)

  • For your References section:

    Structure of the dynein-2 complex and its assembly with intraflagellar transport trains. Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, Roberts AJ. Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y. 10.1038/s41594-019-0286-y PubMed 31451806
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