WDR34
(Plasmid #132526)

• Purpose: Human WDR34, codon optimised for Sf9 expression.
• Depositing Lab: Andrew Carter
• Publication: Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: Geneva Biotech
• Backbone size w/o insert (bp): 2922
• Total vector size (bp): 4527
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: WDR34
• Alt name: WD repeat-containing protein 34
• Species: H. sapiens (human)
• Insert Size (bp): 1605
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: WDR34 (a.k.a. DIC5, FAP133, SRTD11, bA216B9.3)

Resource Information

• Supplemental Documents:
  • p1485_WDR34.gb
• A portion of this plasmid was derived from a plasmid made by: Cloned from genes synthesised by Epoch, codon optised for insect cell expression.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.