His-ZZ-TEV-SNAPf-DYNC2H1
(Plasmid #132524)

• Purpose: Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
• Depositing Lab: Andrew Carter
• Publication: Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: Geneva Biotech
• Backbone size w/o insert (bp): 2932
• Total vector size (bp): 17001
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DYNC2H1
• Alt name: Dynein 2 Heavy chain 1
• Alt name: DHC2
• Species: H. sapiens (human)
• Insert Size (bp): 14069
• Mutation: Codon optimised for Sf9 expression
• Entrez Gene: DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
• Tags / Fusion Proteins:
  • 8x His (N terminal on insert)
  • ZZ (N terminal on insert)
  • Linker-TEV-linker-TEV (N terminal on insert)
  • Precission cleavage site (N terminal on insert)
  • SNAPf (N terminal on insert)

Resource Information

• Supplemental Documents:
  • p1483_His-ZZ-TEV-SNAPf-DYNC2H1.gb
• A portion of this plasmid was derived from a plasmid made by: Cloned from genes synthesised by Epoch, codon optised for insect cell expression.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.