pLPC FLAG hRap1 delta CT
(Plasmid #13252)

• Depositing Lab: Titia de Lange
• Publication: Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5700
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Repressor Activator Protein 1 delta CT
• Alt name: Rap1 delta CT
• Alt name: Rap1
• Alt name: 1090
• Species: H. sapiens (human)
• Insert Size (bp): 1120
• Mutation: contains hRap1 aa2-270 + 331-399 and the point mutation M5L
• GenBank ID: AF262988
• Entrez Gene: TERF2IP (a.k.a. DRIP5, RAP1)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: TAGGCGTGTACGGTGGGA
• 3′ sequencing primer: CTTGCCAAACCTACAGGT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry