pGEX CnA 2-347
(Plasmid #13251)

• Depositing Lab: Anjana Rao
• Publication: Li et al J Mol Biol. 2004 Oct 1. 342(5):1659-74.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CnA
• Alt name: calcineurin A
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: Amino acids 2-347 (truncated before B binding helix)
• Entrez Gene: PPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry