piHGpd/P82
(Plasmid
#1325)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS303 (based on pBluescript
- Backbone size w/o insert (bp) 5000
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGPD promoter-HSP82
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Alt nameYeast Heat shock protein 82
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Alt nameHSP82
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3800
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MutationWild type gene
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Entrez GeneHSP82 (a.k.a. YPL240C, HSP90)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1, BamH1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid maintained in DH5alpha.
ClaI-NotI fragment from pTGpd/P82 was cloned into pRS303
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
piHGpd/P82 was a gift from Susan Lindquist (Addgene plasmid # 1325 ; http://n2t.net/addgene:1325 ; RRID:Addgene_1325) -
For your References section:
In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56. 10.1073/pnas.94.24.12949 PubMed 9371781