pH6-TEV-mCherry
(Plasmid #132430)

• Purpose: Expresses His6-tagged mCherry in E. coli using T7 promoter
• Depositing Lab: Mark Arbing
• Publication: His6 tagged mCherry (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6071
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic; Psychromonas
• Insert Size (bp): 774
• Mutation: Codon optimized
• Promoter: T7
• Tag / Fusion Protein:
  • N-terminal His6 tag with TEV protease site (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pma507star-mcherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A His6-tagged mCherry is expressed from the T7 promoter hence protein expression requires a DE3 strain. The N-terminal tag can be cleaved with TEV protease. Express at 37C for 3-4 hours using E coli BL21(DE3); yield is reduced with overnight expression at lower temperatures.