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PurposeExpressing Fucci cell cycle indicator in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCCL
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehCdt, hGeminin
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCCL-CellCycle was a gift from John M. Greally (Addgene plasmid # 132429 ; http://n2t.net/addgene:132429 ; RRID:Addgene_132429) -
For your References section:
Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains. Sato H, Wu B, Delahaye F, Singer RH, Greally JM. J Cell Biol. 2019 Jun 3;218(6):1810-1823. doi: 10.1083/jcb.201811109. Epub 2019 May 20. 10.1083/jcb.201811109 PubMed 31110057