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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
(Plasmid #132426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    white+attB
  • Backbone size w/o insert (bp) 7860
  • Total vector size (bp) 15444
  • Vector type
    Insect Expression
  • Selectable markers
    mini-white, GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Ubiq promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1865

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    firefly luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    1650
  • GenBank ID

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    T2A-eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    774

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Renilla Luciferase-SV40-P10
  • Species
    Synthetic
  • Insert Size (bp)
    1855

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    reversed ubiq promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1865

Cloning Information for Gene/Insert 5

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OA-1052B (Ubiq-Fluc_Ubiq-Rluc) was a gift from Omar Akbari (Addgene plasmid # 132426 ; http://n2t.net/addgene:132426 ; RRID:Addgene_132426)

  • For your References section:

    Programmable RNA Targeting Using CasRx in Flies. Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS. CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. 10.1089/crispr.2020.0018 PubMed 32584145
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