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Addgene

OA-1050K (firefly luciferase)
(Plasmid #132422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    white+attB
  • Backbone size w/o insert (bp) 7860
  • Total vector size (bp) 8167
  • Vector type
    Insect Expression
  • Selectable markers
    mini-white

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    firefly Luciferase gRNA array
  • gRNA/shRNA sequence
    CAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACGTGTACATCGACTGAAATCCCTGGTAATCCCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACCAGAGCGACACCTTTAGGCAGACCAGTAGACAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACGGCAACCGCTTCCCCGACTTCCTTAGAGAGCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACCGTCGAAGATGTTGGGGTGTTGGAGCAAGACAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAAC
  • Species
    D. melanogaster (fly)
  • Promoter U6-3

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OA-1050K (firefly luciferase) was a gift from Omar Akbari (Addgene plasmid # 132422 ; http://n2t.net/addgene:132422 ; RRID:Addgene_132422)

  • For your References section:

    Programmable RNA Targeting Using CasRx in Flies. Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS. CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. 10.1089/crispr.2020.0018 PubMed 32584145
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