OA-1050S (UASt-dCasRx)
(Plasmid
#132419)
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PurposeExpress dCasRx under UASt promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 132419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepiggyBac
- Backbone size w/o insert (bp) 7733
- Total vector size (bp) 11313
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Vector typeInsect Expression
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Selectable markersdsRed
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCasRx
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SpeciesRuminococcus flavefaciens
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Insert Size (bp)2898
- Promoter UASt
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameUASt+hsp70
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SpeciesD. melanogaster (fly)
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Insert Size (bp)572
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OA-1050S (UASt-dCasRx) was a gift from Omar Akbari (Addgene plasmid # 132419 ; http://n2t.net/addgene:132419 ; RRID:Addgene_132419) -
For your References section:
Programmable RNA Targeting Using CasRx in Flies. Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS. CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. 10.1089/crispr.2020.0018 PubMed 32584145
