OA-1050E (Ubiq-CasRx)
(Plasmid
#132416)
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PurposeExpress CasRx under Ubiquitin-63E promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132416 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepiggyBac+ubiq
- Backbone size w/o insert (bp) 9680
- Total vector size (bp) 12578
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Vector typeInsect Expression
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Selectable markersdsRed
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx
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SpeciesRuminococcus flavefaciens
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Insert Size (bp)2898
- Promoter Ubiq
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OA-1050E (Ubiq-CasRx) was a gift from Omar Akbari (Addgene plasmid # 132416 ; http://n2t.net/addgene:132416 ; RRID:Addgene_132416) -
For your References section:
Programmable RNA Targeting Using CasRx in Flies. Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS. CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. 10.1089/crispr.2020.0018 PubMed 32584145