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Addgene

FRESCA-syntide
(Plasmid #132415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5475
  • Total vector size (bp) 7476
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRESCA-syntide
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2001
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GATCCGGCGGCGCCGGCGGCGGCccgctggcgcgcgccctgaccgtggcgggcctgccgggcaaaaaaGGC
  • 3′ sequencing primer GGCCGCCttttttgcccggcaggcccgccacggtcagggcgcgcgccagcggGCCGCCGCCGGCGCCGCCG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Megan West, UMass-Amherst

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRESCA-syntide was a gift from Margaret Stratton (Addgene plasmid # 132415 ; http://n2t.net/addgene:132415 ; RRID:Addgene_132415)

  • For your References section:

    FRET-based sensor for CaMKII activity (FRESCA): A useful tool for assessing CaMKII activity in response to Ca(2+) oscillations in live cells. Ardestani G, West MC, Maresca TJ, Fissore RA, Stratton MM. J Biol Chem. 2019 Jun 14. pii: RA119.009235. doi: 10.1074/jbc.RA119.009235. 10.1074/jbc.RA119.009235 PubMed 31201271
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