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Addgene

CS2-H2B-EGFP
(Plasmid #132414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS-H2B-mRFP1
  • Backbone manufacturer
    Megason lab (Addgene #53745)
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 5293
  • Modifications to backbone
    The RFP sequence in the original vector was replaced with EGFP
  • Vector type
    Synthetic mRNA production

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1110
  • GenBank ID
    NC_025025.1 NC_000001.11
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GTCGGAGCAAGCTTGATTTAGGTGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EGFP was from a plasmid created by Carl-Phillipe Heisenberg

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CS2-H2B-EGFP was a gift from David Kimelman (Addgene plasmid # 132414 ; http://n2t.net/addgene:132414 ; RRID:Addgene_132414)
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