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Purposeoverexpression in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmRuby3-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby3
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SpeciesSynthetic
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Mutationno
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmRuby3-24xGCN4 was a gift from Ling-Ling Chen (Addgene plasmid # 132413 ; http://n2t.net/addgene:132413 ; RRID:Addgene_132413) -
For your References section:
Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems. Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL. Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. 10.1016/j.molcel.2019.10.024 PubMed 31757757