pLPC myc hPOT1 delta OB
(Plasmid #13241)

• Depositing Lab: Titia de Lange
• Publication: Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5700
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protection of Telomeres 1 delta OB
• Alt name: POT1 delta OB
• Alt name: 2827
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• GenBank ID: FLJ11073 POT1 delta OB
• Entrez Gene: POT1 (a.k.a. CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TAGGCGTGTACGGTGGGA
• 3′ sequencing primer: CTTGCCAAACCTACAGGT

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry