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Addgene

pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
(Plasmid #132402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-EF1a-IRES-puro
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dPspCas13b
  • Species
    Prevotella sp. P5-125
  • Mutation
    H133A; H1059A
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is prone to recombination. Test multiple colonies to ensure the full plasmid is isolated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag was a gift from Ling-Ling Chen (Addgene plasmid # 132402 ; http://n2t.net/addgene:132402 ; RRID:Addgene_132402)

  • For your References section:

    Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems. Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL. Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. 10.1016/j.molcel.2019.10.024 PubMed 31757757
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