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pOlyA(E69A)
(Plasmid #132392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132392 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21c+
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    BL21 (DE3) pLysS E. coli-competent cells used for protein production. Growth at 37°C until O.D. of 0.4 - 0.8. Then, expression induced with 1 mM IPTG at 18°C for 16 h
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ostreolysin A point mutant (E69A)
  • Alt name
    OlyA(E69A)
  • Species
    Pleurotus ostreatus
  • Insert Size (bp)
    480
  • Mutation
    Mutations: C62S, E69A, C94S, S151C
  • GenBank ID
  • Tag / Fusion Protein
    • His6 tag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOlyA(E69A) was a gift from Arun Radhakrishnan (Addgene plasmid # 132392 ; http://n2t.net/addgene:132392 ; RRID:Addgene_132392)

  • For your References section:

    Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes. Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan A. Cell. 2019 Feb 21;176(5):1040-1053.e17. doi: 10.1016/j.cell.2018.12.042. Epub 2019 Jan 31. 10.1016/j.cell.2018.12.042 PubMed 30712872
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